Journal:

Article Title: Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc?RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression

doi: 10.1128/JVI.77.21.11332-11346.2003

Figure Lengend Snippet: MV-N binding on different cell types

Article Snippet: The cell lines 3D1 (murine cortical TEC), HeLa (epithelial human carcinoma from cervix), MeWo (fibroblastic human melanoma from lymph node), NIH 3T3 (mouse fibroblasts from embryo), Jurkat (human acute T-cell leukemia), and Vero (monkey fibroblast from kidney) were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: Binding Assay, Inhibition, Expressing

Journal: Frontiers in Pharmacology

Article Title: Lymph Nodes-On-Chip: Promising Immune Platforms for Pharmacological and Toxicological Applications

doi: 10.3389/fphar.2021.711307

Figure Lengend Snippet: In vitro engineering of human Lymph node-on-a-Chip. (A) Schematic of the human native lymph node. (B) Requirements for in vitro biomimetic lymph node-on-chip. Requirements include: Biomimetic scaffold on which cells can be seeded or grown, cellular components (immune cells and stromal cells), appropriate extracellular matrix components, dynamic flow conditions, key features of the native lymph node including compartmentalization.

Article Snippet: An attempt to model the human lymph node is a commercial perfusion bioreactor system named the Human Artificial Lymph Node reactor (HuALN ® , ProBioGen AG, Berlin, Germany) or HIRIS TM III bioreactor ( ) .

Techniques: In Vitro

Journal: Frontiers in Pharmacology

Article Title: Lymph Nodes-On-Chip: Promising Immune Platforms for Pharmacological and Toxicological Applications

doi: 10.3389/fphar.2021.711307

Figure Lengend Snippet: Summary of existing attempts to develop in vitro lymph nodes on-chip.

Article Snippet: An attempt to model the human lymph node is a commercial perfusion bioreactor system named the Human Artificial Lymph Node reactor (HuALN ® , ProBioGen AG, Berlin, Germany) or HIRIS TM III bioreactor ( ) .

Techniques: In Vitro, Scaffolding, Chemotaxis Assay, Diffusion-based Assay, Derivative Assay, Migration, Adhesive, Blocking Assay, Expressing, Activation Assay, Shear, Purification, Vaccines, Immunopeptidomics

Journal: bioRxiv

Article Title: Tissue-wide profiling of human lungs reveals spatial sequestration of macrophages in tuberculosis

doi: 10.1101/2025.04.11.648467

Figure Lengend Snippet: (A) Heatmap showing the relative abundance of CD4 + (top) and CD8 + (bottom) T cell sub-populations in each lesion type. Rows are ordered based on the similarity in marker expression patterns. N, the border of necrotic lesions. (B) Representative OPAL multi-plex image illustrating a germinal center-like structure in a B3-type lesion. The image with merged DAPI, CD20, BCL6, and PNAd is shown. Arrows indicate PNAd + high endothelial venules and arrow heads indicate BCL6 + B cells. (C) Paired analysis comparing the spatial association relationships between B cells and Tfh (C10) or proinflammatory CD4 + T cells (C08) in all B3-type lesions. Each line indicates a lesion. The level of statistical significance is determined using Wilcoxon signed-rank test, **** P < 0.0001. (D) Cell type pheno-map of a representative B3-type lesion with cell type indicated. (E) Scatter plots showing the spatial centrality index (immCPI) of Tfh (C10), proinflammatory CD4 + T cells (C08), and proinflammatory CD8 + T cells (C11) in all B3-type lesions ( n = 110). The level of statistical significance is determined using a Mann-Whitney U test, **** P < 0.0001. (F) Heatmap showing the median value of pair-wised spatial association relationships between CD4 + and CD8 + T cell subsets in B3 (left) and T (right) type lesions. (G) Column scatter plot showing spatial relationship between macrophages and CD4 + and CD8 + T cell subtypes in T-type lesions. (H) Heatmap indicating statistical significance from post hoc multi-group comparison results in (G) using a One-way ANOVA test, the level of significance was set at P = 0.05.

Article Snippet: Next, tissue sections were incubated for 1 hour at room temperature with primary antibodies against CD20 (1:100, L26, Abcam), CD4 (1:100, EPR6855, Abcam), and overnight under 4°C with BCL6 (1:100, EPR11410-43, Abcam), and PNAd (1:200, MECA-79, Novus Biologicals).

Techniques: Marker, Expressing, MANN-WHITNEY, Comparison

Journal: Clinical and Developmental Immunology

Article Title: Modulatory Effect of 1,25-Dihydroxyvitamin D 3 on IL1 β -Induced RANKL, OPG, TNF α , and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A

doi: 10.1155/2013/160123

Figure Lengend Snippet: Effect of 1,25(OH) 2 D 3 on IL β -induced TNF α and IL-6 production in MH7A. MH7A cells were stimulated with 20 ng/mL IL1 β and then treated with different concentrations of 1,25(OH) 2 D 3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h. The effects of 1,25(OH) 2 D 3 on IL β -induced TNF α (a) and IL-6 (b) mRNA expression in MH7A was analyzed by real-time PCR, and the production of IL-6 in supernatants was shown in (c). The data shown are the mean ± SD for three independent experiments, each in triplicate. * P < 0.05 compared to cells cultured with IL-1 β alone. ** P < 0.01 compared to cells cultured with IL-1 β alone.

Article Snippet: Cytokine production was determined in cell culture supernatants using ELISA specific for human IL-6 and TNF α (BOSTER-BIO; Wuhan; China) following manufacturer's guidelines.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture